Richard B Jones
Research Summary / Selected Publications
We are utilizing protein micro-array, mass spectrometric, and cell biological tools to query both the theoretical biophysical nature of protein-protein interaction connectivity as well as the dynamics of cellular protein abundance, post-translational modification, and interaction connectivity. Our goal is to gain a better understanding of the modular signaling molecules whose location, abundance, and modification state underlie cell growth, migration, differentiation, and cell death: These processes lie at the heart of cancer biology and an understanding of these processes at the systems level should enable the identification of many new therapeutic targets.
We are utilizing protein micro-array, mass spectrometric, and cell biological tools to query both the theoretical biophysical nature of protein-protein interaction connectivity as well as the dynamics of cellular protein abundance, post-translational modification, and interaction connectivity. Our goal is to gain a better understanding of the modular signaling molecules whose location, abundance, and modification state underlie cell growth, migration, differentiation, and cell death: These processes lie at the heart of cancer biology and an understanding of these processes at the systems level should enable the identification of many new therapeutic targets.
Jones R. B., Gordus, A.G., Krall, J., MacBeath, G. (2006) A Quantitative Protein Interaction Network for the ErbB Receptors Using Protein Microarrays. Nature. 439(7073):168-74.
Newman, E.A., Muh, S.J., Hovhannisyan, R.H., Warzecha, C.C., Jones, R. B., McKeehan, W.L., and Carstens, R.P. (2006) Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays. RNA. 12: 1129-1141.
Luo Y., Cho H.H., Jones R. B., Jin C., McKeehan W. L. (2004) Improved Production of Recombinant Fibroblast Growth Factor 7 (FGF7/KGF) from Bacteria in High Magnesium Chloride. Protein Expression and Purification. 33:326-31.
Ye S., Luo Y., Lu W., Jones R. B., Linhardt R. J., Capila I., Toida T., Kan M., Pelletier H., McKeehan W. L. (2001) Structural Basis for Interaction of FGF-1, FGF-2, and FGF-7 with Different Heparan Sulfate Motifs. Biochemistry 40:14429-14439.
Jones R. B., Carstens R. P, Luo Y., McKeehan W. L. (2001) 5'- and 3'-terminal Nucleotides in the FGFR2 ISAR Splicing Element Core Have Overlapping Roles in Exon IIIb Activation and Exon IIIc Repression. Nucleic Acids Research. 29:3557-3565.
Jones, R. B., Wang, F., Luo, Y., Yu, C., Jin, C., Suzuki, T., Kan, M., McKeehan, W. L. (2001) The Nonsense-Mediated Decay Pathway and Mutually Exclusive Expression of Alternatively Spliced FGFR2IIIb and IIIc mRNAs. Journal of Biological Chemistry. 276:4158-4167.
Jones R. B., Gordus, A.G., Krall, J., MacBeath, G. (2006) A Quantitative Protein Interaction Network for the ErbB Receptors Using Protein Microarrays. Nature. 439(7073):168-74.
Newman, E.A., Muh, S.J., Hovhannisyan, R.H., Warzecha, C.C., Jones, R. B., McKeehan, W.L., and Carstens, R.P. (2006) Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays. RNA. 12: 1129-1141.
Luo Y., Cho H.H., Jones R. B., Jin C., McKeehan W. L. (2004) Improved Production of Recombinant Fibroblast Growth Factor 7 (FGF7/KGF) from Bacteria in High Magnesium Chloride. Protein Expression and Purification. 33:326-31.
Ye S., Luo Y., Lu W., Jones R. B., Linhardt R. J., Capila I., Toida T., Kan M., Pelletier H., McKeehan W. L. (2001) Structural Basis for Interaction of FGF-1, FGF-2, and FGF-7 with Different Heparan Sulfate Motifs. Biochemistry 40:14429-14439.
Jones R. B., Carstens R. P, Luo Y., McKeehan W. L. (2001) 5'- and 3'-terminal Nucleotides in the FGFR2 ISAR Splicing Element Core Have Overlapping Roles in Exon IIIb Activation and Exon IIIc Repression. Nucleic Acids Research. 29:3557-3565.
